Profil Biokimia Darah Akibat Pemberian Ekstrak Akar Burdock (Arctium lappa L.) pada Tikus Putih (Rattus norvegicus)
DOI:
https://doi.org/10.55606/klinik.v5i1.5307Keywords:
Arctium lappa L.., Blood Biochemical Profile, Burdock Root Extract, Rattus norvegicus, White RatsAbstract
Burdock (Arctium lappa L.) is a medicinal plant that contains active compounds such as flavonoids, lignans, and phenolic acids, which act as antioxidants, anti-inflammatory agents, and hepatoprotective substances. This study aimed to evaluate the blood biochemical profile following the administration of burdock root extract in white rats (Rattus norvegicus). The study was conducted experimentally using eight male and female rats, divided into four groups: a negative control group (aquadest) and three treatment groups receiving doses of 150, 300, and 450 mg/kgBB orally for 28 days. Blood biochemical parameters were examined on day 36, including albumin, total protein, globulin, glucose, urea, amylase, cholesterol, ALT, ALP, total bilirubin, creatinine, and creatine kinase. The results showed that most biochemical parameters remained within the normal range, although significant changes were observed in some groups. Urea, amylase, and total bilirubin levels remained stable, while total protein, globulin, and ALP levels tended to be higher in female rats. Elevated glucose, cholesterol, and ALT levels in most treatment groups indicated potential disturbances in carbohydrate and lipid metabolism, while increased creatinine levels in some groups suggested possible nephrotoxic effects. Administration of burdock root extract influenced the biochemical blood profile, particularly related to glucose and lipid metabolism as well as liver function. Further studies are needed to explore the underlying mechanisms and evaluate the long-term safety of burdock root extract.
References
Akrianto, A. (2021). Pengaruh sterilisasi terhadap kadar alkaline phosphatase (ALP) pada tikus putih (Rattus norvegicus). Angewandte Chemie International Edition, 6(11), 951–952.
Alhusaini, A., Fadda, L., Hasan, I. H., Ali, H. M., El Orabi, N. F., Badr, A. M., Zakaria, E., Alenazi, A. M., & Mahmoud, A. M. (2019). Arctium lappa root extract prevents lead-induced liver injury by attenuating oxidative stress and inflammation, and activating Akt/GSK-3β signaling. Antioxidants, 8(12), 582. https://doi.org/10.3390/antiox8120582
Anzelita, A., Lubis, A. A., & Yunus, M. (2025). Efek protektif ekstrak jinten hitam (Nigella sativa L.) terhadap gambaran histopatologi ginjal tikus putih (Rattus norvegicus) yang diinduksi tetrasiklin. Scientific Progress Research, 2(2), 1–11. https://doi.org/10.47134/scpr.v2i2.3975
Faizal, I. A., Puspodewi, D., & Aji, A. P. (2024). Uji toksisitas subkronik nanoemulsi akar burdock (Arctium lappa L.) berdasarkan histopatologi pada tikus albino Rattus noverticus (strain Wistar). Indonesian Journal of Innovation Multidisciplinary Research, 2(2), 331–342. https://doi.org/10.69693/ijim.v2i2.140
Fauziyah, R., Widyasanti, A., & Rosalinda, S. (2022). Perbedaan metode ekstraksi terhadap kadar sisa pelarut dan rendemen total ekstrak bunga telang (Clitoria ternatea L.). Kimia Padjadjaran, 1(1), 18–25.
Fitria, L., Lukitowati, F., & Kristiawati, D. (2019). Nilai rujukan untuk evaluasi fungsi hati dan ginjal pada tikus (Rattus norvegicus Berkenhout, 1769) galur Wistar. Jurnal Pendidikan Matematika dan IPA, 10(2), 81–89. https://doi.org/10.26418/jpmipa.v10i2.34144
Hermawan, I. P., & Mudji, E. H. (2023). Total protein value of stray cats and domestic pet cats in Surabaya City. Jurnal Medika Veterinaria, 16(1), 15–20. https://doi.org/10.21157/j.med.vet..v16i1.20320
Hou, B., Wang, W., Gao, H., Cai, S., & Wang, C. (2018). Effects of aqueous extract of Arctium lappa L. roots on serum lipid metabolism. Journal of International Medical Research, 46(1), 158–167. https://doi.org/10.1177/0300060517716341
Jeharu, S. A., Putra, I. G., & Widayanti, N. P. (2020). Pengaruh pemberian variasi dosis rifampisin terhadap kadar gamma glutamyl transferase dan alkaline phosphatase pada tikus putih galur Wistar. Bali International Scientific Forum, 1(1), 25–34. https://doi.org/10.34063/bisf.v1i1.129
Karwiti, W., & Umizah, L. P. (2023). Gambaran kadar ureum pada pasien gagal ginjal kronik yang menjalani hemodialisa. Journal of Medical Laboratory and Science, 3(2). https://doi.org/10.36086/medlabscience.v3i2
Merthayasa, J. D., Devi Jayanti, P. D., Indarjulianto, S., Permana, R. H., Destinanda, N. L., & Wijayanti, A. D. (2019). Pengaruh pemberian serum albumin manusia terhadap kadar albumin dalam darah pada anjing dengan status hipoalbuminemia. Jurnal Sain Veteriner, 37(1), 34–41. https://doi.org/10.22146/jsv.48513
Mondal, S. C., & Eun, J. B. (2022). Mechanistic insights on burdock (Arctium lappa L.) extract effects on diabetes mellitus. Food Science and Biotechnology, 31(8), 999–1008. https://doi.org/10.1007/s10068-022-01091-2
Moro, T., & Clerici, M. (2021). Burdock (Arctium lappa L.) roots as a source of inulin-type fructans and other bioactive compounds: Current knowledge and future perspectives for food and non-food applications. Food Research International, 141, 109889. https://doi.org/10.1016/j.foodres.2020.109889
Naim, M. R., Sulastri, S., & Hadi, S. (2019). Gambaran hasil pemeriksaan kadar kolesterol pada penderita hipertensi di RSUD Syekh Yusuf Kabupaten Gowa. Jurnal Media Laboran, 9(2), 33–38.
Nasution, D. P. (2022). Gambaran kadar enzim aspartat aminotransferase (AST) dan enzim alanin aminotransferase (ALT) pada pasien penderita sirosis hati di Rumah Sakit Efarina Etaham Berastagi. Jurnal Ilmiah Multidisiplin, 1(5), 992–996. https://doi.org/10.36760/jp.v5i2.315
Patel, S., Patel, S., Kotadiya, A., Patel, S., Shrimali, B., Joshi, N., Patel, T., Trivedi, H., Patel, J., Joharapurkar, A., & Jain, M. (2024). Age-related changes in hematological and biochemical profiles of Wistar rats. Laboratory Animal Research, 40(1), 1–12. https://doi.org/10.1186/s42826-024-00194-7
Ramadhan, B., & Wikandari, P. R. (2021). Aktivitas enzim amilase dari bakteri asam laktat (Karakteristik dan aplikasi): Review artikel. Unesa Journal of Chemistry, 10(2), 109–120. https://doi.org/10.26740/ujc.v10n2.p109-120
Skowronska, W., Granica, S., Dziedzic, M., Kurkowiak, J., Ziaja, M., & Bazylko, A. (2021). Comparison of anti-lipoxygenase and antioxidant activity from roots. Plants, 10(78), 1–10. https://doi.org/10.3390/plants10010078
Tuaputimain, S., Lestari, E., & Sukeksi, A. (2020). Perbedaan kadar kreatinin darah sebelum dan sesudah aktivitas fisik. Jurnal Labora Medika, 4(2), 47–51.
Wati, R., Rustiah, W., Rauf, D., & Rais, K. (2023). Gambaran pemeriksaan kadar bilirubin total pada pengonsumsi minuman beralkohol. Jurnal Media Analis Kesehatan, 14(1), 1–6. https://doi.org/10.32382/mak.v14i1.3198.
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