Karakterisasi Aktivitas Antioksidan Ekstrak Etanol Bawang Lanang (Allium sativum L.) Melalui Uji DPPH
DOI:
https://doi.org/10.55606/klinik.v4i2.3940Keywords:
Allium sativum, antioxidant activity, DPPHAbstract
Purpose: This study aimed to evaluate the antioxidant activity and identify secondary metabolite compounds of the ethanol extract from lanang onion bulbs (Allium sativum L.) against DPPH free radicals. Methodology/approach: Extraction was performed by maceration using 95% ethanol, followed by fractionation with n-hexane, ethyl acetate, and water. Antioxidant activity was tested qualitatively by thin-layer chromatography (TLC) and quantitatively by the DPPH method using a UV-Vis spectrophotometer. Phytochemical screening was carried out to identify bioactive compound content. Results/findings: The ethanol extract and ethyl acetate fraction exhibited strong antioxidant activity with IC₅₀ values of 13.85 ppm and 7.27 ppm, respectively. Phytochemical tests revealed the presence of flavonoids, saponins, alkaloids, tannins, steroids, and triterpenoids in each fraction. Limitations: This study was limited to in vitro testing and did not include comprehensive toxicity or in vivo analysis. Contribution: This research contributes to the development of natural antioxidants in pharmacology, phytochemistry, traditional medicine, and functional food industries. The results are useful for researchers, academics, and herbal or health-related industries.
References
Amin, S. (2014). Analisis minyak atsiri umbi bawang putih (Allium sativum Linn.) menggunakan kromatografi gas-spektrometer massa. Jurnal Kesehatan Bakti Tunas Husada, 13(2), 70–75.
Amin, S. (2015). Uji aktivitas antioksidan umbi bawang lanang (Allium sativum) terhadap radikal bebas DPPH (1,1-difenil-2-pikrilhidrazil). Jurnal Kesehatan Bakti Tunas Husada, 15(1), 20–26.
Amin, S., & Ramdhani, R. (2015). Uji aktivitas antioksidan dan telaah fitokimia Sargassum crassifolium J.G. Agardh. Jurnal Kesehatan Bakti Tunas Husada, 14(2), 115–122.
Amirna, N. (2024). antioxidant capacity of ethanol extract of garlic (Allium sativum) in vitro. Makassar Pharmaceutical Science Journal (MPSJ), 1(4), 251-259.
Armin, S., & Dewi, S. R. (2011). Identifikasi senyawa flavonoid pada tanaman obat tradisional. Jurnal Farmasi Indonesia, 6(1), 45–49.
Azhar, S. F., & Yuliawati, K. M. (2021). Pengaruh waktu aging dan metode ekstraksi terhadap aktivitas antioksidan black garlic yang dibandingkan dengan bawang putih (allium sativum L.). Jurnal Riset Farmasi, 16-23.
Azizah, Z., Yani, P., & Yetti, R. D. (2020). Antioxidant activity ethanol extract of garlic (Allium sativum L.) and black garlic. Int. J. Res. Rev, 7(9), 94-103.
Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199–1200.
Halliwell, B., & Gutteridge, J. M. C. (2007). Free radicals in biology and medicine (4th ed.). Oxford: Oxford University Press.
Handarini, R. (2014). Analisis kandungan saponin dalam ekstrak daun binahong (Anredera cordifolia). Surabaya: Universitas Airlangga.
Kusbandari, A., & Susanti, H. (2017). Kandungan beta karoten dan aktivitas penangkapan radikal bebas terhadap DPPH pada blewah. Jurnal Farmasi Sains dan Komunitas, 14(1), 37–42.
Molyneux, P. (2004). The use of the stable free radical DPPH for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2), 211–219.
Prakash, A., Rigelhof, F., & Miller, E. (2001). Antioxidant activity. Medallion Laboratories Analytical Progress, 19(2), 1–4.
Prasonto, D., Riyanti, E., & Gartika, M. (2017). Uji aktivitas antioksidan ekstrak bawang putih (Allium sativum). Odonto: Dental Journal, 4(2), 122–128.
Umiyah, U., & Umayah, E. (2007). Uji aktivitas antioksidan ekstrak kenitu (Chrysophyllum cainito L.) terhadap radikal bebas DPPH. Berkala Penelitian Hayati, 13(1), 45–50.
Valko, M., Izakovic, M., Mazur, M., Rhodes, C. J., & Telser, J. (2006). Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biological Interactions, 160(1), 1–40.
Wardatun, S., Husni, E., & Arief, M. (2016). Uji aktivitas antidiabetes ekstrak etanol daun salam (Syzygium polyanthum) menggunakan metode Nelson-Somogyi. Jurnal Farmasi Galenika, 2(2), 105–112.
Wilujeng, D. T., & Anggarani, M. A. (2021). PENENTUAN FENOLIK TOTAL, FLAVONOID TOTAL, DAN AKTIVITAS ANTIOKSIDAN EKSTRAK BAWANG LANANG (Allium sativum L.): DETERMINATION OF TOTAL PHENOLIC, TOTAL FLAVONOIDS, AND ANTIOXIDANT ACTIVITY OF SINGLE BULB GARLIC EXTRACT (Allium sativum L.). UNESA Journal of Chemistry, 10(3), 295-306.
Winarsi, H. (2007). Antioksidan alami dan radikal bebas. Yogyakarta: Kanisius.
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