Efektivitas Ekstrak Etanol Bunga Telang (Clitoria Ternatea L ) sebagai Nefroprotektor terhadap Tikus Putih Jantan (Rattus Norvegicus) yang Diinduksi Gentamisin
DOI:
https://doi.org/10.55606/klinik.v4i1.5147Keywords:
Bunga Telang, Creatinine, Gentamicin, Nephroprotective, Renal HistopathologyAbstract
Nephrotoxicity is a disorder of kidney function that can occur due to exposure to drugs, environmental chemicals, or other toxic substances. One aminoglycoside antibiotic known to have nephrotoxic effects is gentamicin, which is often used in the treatment of bacterial infections, but can cause kidney damage if used in high doses or for long periods. Therefore, it is important to find agents that can protect the kidneys from the side effects of this drug. Butterfly Pea Flower (Clitoria ternatea L.) is a plant known to contain secondary metabolites with antioxidant activity that have the potential to act as nephroprotective agents. These compounds can help combat oxidative stress that occurs in the kidneys due to exposure to toxic substances. This study aims to determine the optimal dose of Butterfly Pea Flower ethanol extract in protecting the kidneys of white rats from gentamicin-induced damage. A total of 25 male white mice were divided into five treatment groups: a normal control group (given distilled water), a negative control group (given only gentamicin 100 mg/kgBW), and three test groups given Butterfly Pea Flower extract at doses of 100, 200, and 400 mg/kgBW. Evaluation was carried out by measuring serum creatinine levels as an indicator of kidney function and histopathological analysis of kidney tissue to assess structural damage to the rats' kidneys. The results showed that administration of gentamicin caused a significant increase in serum creatinine levels and relative kidney weight, indicating acute kidney damage. Conversely, administration of Butterfly Pea Flower extract, especially at a dose of 400 mg/kgBW, provided the most effective protection against kidney damage. This was indicated by a decrease in creatinine levels approaching normal control values and improvement in histological damage to kidney tissue, such as repair of the structure of kidney tubules damaged by the effects of gentamicin.
References
Abd-Elkareem, M., Soliman, M., Abd El-Rahman, M. A. M., & Abou Khalil, N.S. (2022). Effect of Nigella sativa L. Seed on the Kidney of Monosodium Glutamate Challenged Rats. Frontiers in Pharmacology, 13. https://doi.org/10.3389/fphar.2022.789988
Alamor, M.Y. (2020). Physiological and histopathological study on the influence of Ocimum basilicum leaves extract on thioacetamide-induced nephrotoxixity in make rats. Saudi Journal of Biological Sciences, 27(7), 1843-1849. https://doi.org/10.1016/j.sjbs.2020.05.034
Amriani, A., Fitrya, Novita, R. P., & Caniago, D. (2021). Uji aktivitas antidiabetes ekstrak etanol akar kabau (Archidendron bubalinum (Jack) I.C. Nielsen) terhadap tikus putih jantan yang diinduksi diet tinggi lemak dan fruktosa. Jurnal Penelitian Sains, 23(1), 102 109. https://doi.org/10.56064/jps.v23i2.635
Azzahra, A. J., Fikayuniar, L., Amallia, S., Anisa, M. A., Sagala, B. C., & Irawan, L. (2023). Skrinning fitokimia serta uji karakteristik simplisia dan ekstrak bunga telang (Clitoria ternatea l.) Dengan berbagai metode. Jurnal Ilmiah Wahana Pendidikan, 9(15), 308-320.
Cahyani, E. D., Prasetya, R. A., Ma'rifah, I., Widia, D. N. T., & Sri, T. (2022) In vivo nephroprotective effect of herbal plants towards gentamicin-induced nephrotoxicity: A literature review Kajian literatur efek nefroprotektif tanaman herbal terhadap nefrotoksisitas yang diinduksi gentamisin secara in vivo https://doi.org/10.20885/jif.vol18.iss2.art17
Cahyaningsih, E., Yuda, P. E. S. K., & Santoso, P. (2019). Skrining fitokimia dan uji aktivitas antioksidan ekstrak etanol bunga telang (Clitoria ternatea L.) dengan metode spektrofotometri uv-Vis. Jurnal Ilmiah Medicamento, 5(1).EduMatSains, 4 (2). 111-124. https://doi.org/10.36733/medicamento.v5i1.851
Dewi, L. I., Suprijono, M. A., & Antari, A. D. PENGARUH EKSTRAK BUNGA TELANG (CLITORIA TERNATEA L.) TERHADAP NEKROSIS TUBULUS GINJAL. Jurnal Ilmiah Sultan Agung, 3(2), 164-173.
Fatihah rahma Dzikriyah. (2024). Uji aktivitas Antiinflamasi Ekstrak etanol bunga (telang Clitoria ternatea L) pada tikus putih jantan. Fakultas Matematika Dan Ilmu Pengetahuan, Universitas Pakuan. herbal melalui mukosistas. Risenologi (Jurnal Sains, Teknologi, Sosial, Pendidikan, dan Bahasa, 4(2), 65-73
JAENUDIN, J. (2019).UJI AKTIVITAS KACANG GUDE (Cajanus cajan (Linn.) Huth) SEBAGAI NEFROPROTEKTOR PADA TIKUS JANTAN PUTIH GALUR WISTAR (Rattus novergicus) (Doctoral dissertation, STIKes BTH Tasikmalaya). Journal of functional food and nutrition, 1(2), 1-23.
Jannah, D. R., & Budijastuti, W. (2022). Gambaran Histopatologi Toksisitas Ginjal Tikus Jantan (Rattus norvegicus) yang diberi Sirup Umbi Yakon (Smallanthus sonchifolius). LenteraBio: Berkala Ilmiah Biologi, 11(2), 238-246. https://doi.org/10.26740/lenterabio.v11n2.p238-246
Kopon, A.M., Kowalski, R., & Nowak, D. (2020). Validation of Dragendorff's reagent method for qualitative alkaloid screening in plant extracts. Journal of Phytochemical Analysis, 35(4), 112-125.
Marpaung, A. M. (2020). Tinjauan manfaat bunga telang (Clitoria ternatea L.) bagi Kesehatan manusia. https://doi.org/10.33555/jffn.v1i2.30
Muda, G. J., Arjana, A. A. G., Berata, I. K., & Merdana, I. M. (2020). Perubahan Histopatologi Hati Tikus Putih yang diberikan Ekstrak Etanol Sarang Semut dan Gentamisin. Buletin Veteriner Udayana Volume, 12(1), 7-12. https://doi.org/10.24843/bulvet.2020.v12.i01.p02
Nessa. (2025). Pengaruh pemberian ekstrak etanol rambut jagung (Zea mays) terhadap gangguan fungsi ginjal tikus putih jantan. Jurnal Akademi Farmasi Prayoga, 10(1), 34-41. https://doi.org/10.56350/jafp.v10i1.16
Perdanawati, A. L., Ratnaningtyas, N. I., & Hernayanti, H. (2022). Potensi ekstrak etil asetat Coprinus comatus terhadap kadar ureum dan kreatinin pada tikus putih model diabetes. BioEksakta: Jurnal Ilmiah Biologi Unsoed, 4(3) https://doi.org/10.20884/1.bioe.2021.3.3.4239
Purwanto, UMS, & Aprilia, K. (2022). Aktivitas Antioksidan Ekstrak Telang (Clitoria ternatea L.) dalam Menghambat Peroksidasi Lipid. Current Biochemistry , 9 (1), 26-37. https://doi.org/10.29244/cb.9.1.3
Pusmarani, J., Ifaya, M., & Khalid, N. H. A. (2023). Efek Nefroprotektif Kulit Pisang Raja (Musa paradisiaca var. Sapientum) terhadap Kadar Kreatinin Tikus yang Diinduksi Parasetamol. Jurnal Mandala Pharmacon Indonesia, 9(1), 119-124. https://doi.org/10.35311/jmpi.v9i1.320
Pusporini, R., & Fuadiyah, D. (2020). Mengenal Pereda Nyeri Dalam Kedokteran Gigi. Universitas Brawijaya Press
Rajak, Z. F., Loho, L., & Lintong, P. (2016). Gambaran histopatologik ginjal wistar yang diberi ekstrak binahong pasca pemberian gentamisin. eBiomedik, 4(2). https://doi.org/10.35790/ebm.4.2.2016.13911
Silitonga, M., Gultom, E. S., & Nugrahalia, M. (2020). The Effect of Plectranthus amboinicus Lour Spreng Ethanolic Extract on Relative Organ, Body Weights Changes, and Hematology Profile in Wistar Rats Treated with 7,12Dimethylbenz(a)anthracene. Journal of Physics: Conference Series, 1462, 012001. https://doi.org/10.1088/1742-6596/1462/1/012001
Singh, N. K., Garabadu, D., Sharma, P., Shrivastava, S. K., & Mishra, P. (2018). Anti-allergy and anti-tussive activity of Clitoria ternatea L. in experimental animals. Journal of Ethnopharmacology, 224, 15-26. https://doi.org/10.1016/j.jep.2018.05.026
Sujono, T. A., & Rizki, F. A. (2020). Efek nefroprotektif ekstrak etanol bawang putih (Allium sativum L.) pada tikus yang diinduksi gentamisin. Pharmacon: Jurnal Farmasi Indonesia, 1-9. https://doi.org/10.23917/pharmacon.v0i0.10510
Zain, D. N., Pebiansyah, A., & Aprilia, A. Y. (2021). Aktivitas Nefroprotektif Ekstrak Etanol Bunga Telang (Clitoria Ternatea L.) Terhadap Tikus Yang Diinduksi Parasetamol. Pharmacoscript, 4(2), 185-193 https://doi.org/10.36423/pharmacoscript.v4i2.744
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Jurnal Ilmiah Kedokteran dan Kesehatan
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
